说明书
MSDS产品描述：Rabbit polyclonal antibody to LDHA免疫原：Recombinant full length protein of human LDHA纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)基因名称：LDHA相关名称：L-lactate dehydrogenase A chain; LDH-A; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59
基因编号(人)：
3939;
基因编号(小鼠)：
16828;
基因编号(大鼠)：
24533;
蛋白编号(人)：
P00338;
蛋白编号(小鼠)：
P06151;
蛋白编号(大鼠)：
P04642;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LDHA expression in MCF7 (A), Jurkat (B), NIH3T3 (C), mouse skeletal muscle (D) whole cell lysates. (Predicted band size: 26; 30; 36; 39 kD; Observed band size: 37 kD) -
Immunofluorescent analysis of LDHA staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Differential Expression of Lonp1 Isoforms in Cancer Cells