说明书
MSDS产品描述：Rabbit polyclonal antibody to CCT3免疫原：Recombinant full length protein of human CCT3纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：CCT3相关名称：CCTG; TRIC5; T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5
基因编号(人)：
7203;
基因编号(小鼠)：
12462;
基因编号(大鼠)：
295230;
蛋白编号(人)：
P49368;
蛋白编号(小鼠)：
P80318;
蛋白编号(大鼠)：
Q6P502;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CCT3 expression in MCF7 (A), THP1 (B), mouse ovary (C), mouse thymus (D), rat spleen (E), rat heart (F) whole cell lysates. (Predicted band size: 56; 60 kD; Observed band size: 60 kD) -
Immunofluorescent analysis of CCT3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.