产品描述：Rabbit polyclonal antibody to Granzyme B免疫原：Recombinant full length protein of human Granzyme B纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)基因名称：GZMB相关名称：CGL1; CSPB; CTLA1; GRB; Granzyme B; C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E 基因编号(人)： 3002; 基因编号(小鼠)： 14939; 基因编号(大鼠)： 100361468; 171528; 蛋白编号(人)： P10144; 蛋白编号(小鼠)： P04187; 蛋白编号(大鼠)： P18291; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Granzyme B expression in mouse spleen (A), mouse lung (B) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 27 kD)
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  Immunofluorescent analysis of Granzyme B staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.