产品描述:Rabbit polyclonal antibody to CTGF免疫原:KLH-conjugated synthetic peptide of human CTGF纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称:CTGF相关名称:CCN2; HCS24; IGFBP8; Connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Insulin-like growth factor-binding protein 8; IBP-8; IGF-binding protein 8; IGFBP-8
基因编号(人):
1490;
蛋白编号(人):
P29279;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CTGF expression in Hela (A), HepG2 (B) whole cell lysates. (Predicted band size: 35; 38 kD; Observed band size: 38 kD)
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Immunofluorescent analysis of CTGF staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).