产品描述:Rabbit polyclonal antibody to Periplakin免疫原:Recombinant full length protein of human Periplakin纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称:PPL相关名称:KIAA0568; Periplakin; 190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein
基因编号(人):
5493;
基因编号(小鼠):
19041;
蛋白编号(人):
O60437;
蛋白编号(小鼠):
Q9R269;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Periplakin expression in MCF7 (A), mouse lung (B), mouse heart (C) whole cell lysates. (Predicted band size: 204 kD; Observed band size: 205 kD)
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Immunofluorescent analysis of Periplakin staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.