产品描述：Rabbit polyclonal antibody to Bex3免疫原：Recombinant fusion protein of human Bex3. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：NGFRAP1相关名称：BEX3; DXS6984E; NADE; Protein BEX3; Brain-expressed X-linked protein 3; Nerve growth factor receptor-associated protein 1; Ovarian granulosa cell 13.0 kDa protein HGR74; p75NTR-associated cell death executor 基因编号(人)： 27018; 基因编号(小鼠)： 12070; 基因编号(大鼠)： 117089; 蛋白编号(人)： Q00994; 蛋白编号(小鼠)： Q9WTZ9; 蛋白编号(大鼠)： Q6PDU5; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Bex3 expression in MCF7 (A), mouse liver (B) whole cell lysates. (Predicted band size: 11; 12 kD; Observed band size: 24 kD)
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  Immunofluorescent analysis of Bex3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.