产品描述：Rabbit polyclonal antibody to DGAT1免疫原：KLH-conjugated synthetic peptide of human DGAT1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：DGAT1相关名称：AGRP1; DGAT; Diacylglycerol O-acyltransferase 1; ACAT-related gene product 1; Acyl-CoA retinol O-fatty-acyltransferase; ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase 基因编号(人)： 8694; 基因编号(小鼠)： 13350; 基因编号(大鼠)： 84497; 蛋白编号(人)： O75907; 蛋白编号(小鼠)： Q9Z2A7; 蛋白编号(大鼠)： Q9ERM3; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of DGAT1 expression in mouse heart (A) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
• 
  Immunofluorescent analysis of DGAT1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).