产品描述：Human KDM2A knockdown HEK293T cell lysate is engineered by our optimized transduction of the specific shRNA.产品形式：Liquid in 50 mM Tris-HCl, 2% SDS, 10% glycerol, 100 mM DTT, 0.01% Bromophenol blue基因名称：KDM2A相关名称：CXXC8; FBL7; FBXL11; JHDM1A; KIAA1004; Lysine-specific demethylase 2A; CXXC-type zinc finger protein 8; F-box and leucine-rich repeat protein 11; F-box protein FBL7; F-box protein Lilina; F-box/LRR-repeat protein 11; JmjC domain-containing histone demethylation protein 1A; [Histone-H3]-lysine-36 demethylase 1A 基因编号(人)： 22992; 蛋白编号(人)： Q9Y2K7; 质量控制：KD Validation: RT-qPCR使用指导：Thaw the vial on ice or at room temperature. Mix gently by pipetting or brief vortexing. Briefly centrifuge to collect the sample. Load the desired amount of lysate directly onto an SDS-PAGE gel. Perform SDS-PAGE and Western blot analysis using standard laboratory protocols. Note: This lysate has been pre-denatured in SDS sample buffer containing DTT and does not require additional reducing agent or boiling before electrophoresis.样本类型：HEK293T组分：Human KDM2A knockdown HEK293T cell lysate x 1 vial; shControl HEK293T cell lysate x 1 vial储存效期：Shipped at 4°C. Upon delivery aliquot and store at -80°C for one year. Avoid freeze/thaw cycles.

• 
  RT-qPCR analysis of KDM2A knockdown efficiency. Compared with the 293T shRNA control (left), KDM2A mRNA expression in the stable shRNA knockdown cell line (right) decreased, and the knockdown efficiency was 66%.
• 
  RT-qPCR amplification curves. The Ct values of the knockout sample significantly increased, confirming the effective knockdown of KDM2A mRNA. [Red curve (knockdown sample), Blue curve (shRNA control), Purple and Green curve (reference gene GAPDH)]
• 
  RT-qPCR melt curve analysis demonstrated highly specific PCR amplification product without detectable nonspecific amplification or primer-dimer formation. [Red curve (knockdown sample), Blue curve (shRNA control), Purple and Green curve (reference gene GAPDH)]
• 
  This picture presents the raw fluorescence data (relative fluorescence units, RFU) used to generate the melt curves. [Red curve (knockdown sample), Blue curve (shRNA control), Purple and Green curve (reference gene GAPDH)]