产品描述：siRNA to inhibit ZBTB7A expression using RNA interference产品形式：Lyophilized powder基因名称：ZBTB7A相关名称：LRF; ZBTB7; Zinc finger and BTB domain-containing protein 7A; Leukemia/lymphoma-related factor; POZ and Krueppel erythroid myeloid ontogenic factor; POK erythroid myeloid ontogenic factor; Pokemon 基因编号(小鼠)： 16969; 蛋白编号(小鼠)： O88939; 纯度：> 97%使用指导：We recommends transfection with 10 nM - 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For example, resuspend one tube of 5 nmol siRNA oligo in 250 μl of DEPC water to get a final concentration of 20 μM.质量控制：Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.组分：We offers pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZBTB7A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot.储存效期：Shipped at 4 °C. Store at -20 °C for one year.