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CRG1002
  • Representative Standard Curve
产品名称:Bradford Protein Assay Kit
货号:CRG1002
来源:
反应物种:All
实验应用:Protein quantification
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
500 Assays
300
Ship in 3 days
产品描述:Detection and Quantification of Protein Concentration.
检测方法:Colorimetric
检测范围:15.6 μg/ml - 2000 μg/ml
组分:Coomassie Bradford Assay Reagent: 100 ml, stored at 4°C. Standard: 2 mg x 5, Stored at 4°C.
背景应用:Bradford coomassie-binding, colorimetric method for total protein quantitation is one of dye binding methods. Coomassie Briliant Blue G-250 in the free state shows brown, and its maximum light absorbance is 488nm. When Coomassie Briliant Blue G-250 combines with protein, it turns brown into blue, and the maximum absorption is 595 nm. The absorbance at 595nm is proportional to the protein concentrations. Protein binding Coomassie Briliant Blue G-250 reaches a balance in about 2 minutes. The complex remains stable in 1 hour at room temperature. The method has superiority such as simple and convenient operation, sensitive reaction (4 times higher than Lowry Method), etc. Bradford protein assay kit is developed based on Bradford coomassie-binding method. This method has traits of simple and convenient operation, high sensitivity, accurate quantification and stable effect.
储存效期:Shipped and store at 4°C for 1 year.
样本类型:All
检测平台:Microplate reader 595 nm
  • Representative Standard Curve
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