说明书
MSDS产品描述：Mouse monoclonal antibody to GSK3 beta免疫原：Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.稀释比：WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/50 - 1/100)基因名称：GSK3B相关名称：Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
基因编号(人)：
2932;
基因编号(小鼠)：
56637;
基因编号(大鼠)：
84027;
蛋白编号(人)：
P49841;
蛋白编号(小鼠)：
Q9WV60;
蛋白编号(大鼠)：
P18266;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GSK3 beta expression in Hela (A), NIH3T3 (B), C6 (C), rat brain (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD) -
Immunohistochemical analysis of GSK3 beta staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Flow cytometric analysis of GSK3 beta in NIH3T3 cells (green) and negative control (red).