产品描述：Rabbit polyclonal antibody to GPR142免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human GPR142. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), FC (1/10 - 1/50)基因名称：GPR142相关名称：PGR2; Probable G-protein coupled receptor 142; G-protein coupled receptor PGR2 基因编号(人)： 350383; 蛋白编号(人)： Q7Z601; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GPR142 expression in HL60 (A) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 46 kD)
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  Flow cytometric analysis of HL60 cells using Anti-GPR142 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.