说明书
MSDS产品描述：Rabbit polyclonal antibody to GOLGA5免疫原：Recombinant fusion protein of human GOLGA5. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：GOLGA5相关名称：RETII; RFG5; Golgin subfamily A member 5; Cell proliferation-inducing gene 31 protein; Golgin-84; Protein Ret-II; RET-fused gene 5 protein
基因编号(人)：
9950;
基因编号(小鼠)：
27277;
基因编号(大鼠)：
299258;
蛋白编号(人)：
Q8TBA6;
蛋白编号(小鼠)：
Q9QYE6;
蛋白编号(大鼠)：
Q3ZU82;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GOLGA5 expression in HeLa (A), mouse brain (B), mouse heart (C), rat testis (D) whole cell lysates. (Predicted band size: 78; 83 kD; Observed band size: 100; 115 kD) -
Immunofluorescent analysis of GOLGA5 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).