产品描述:Rabbit polyclonal antibody to GNAT3免疫原:Recombinant fusion protein of human GNAT3. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称:GNAT3相关名称:Guanine nucleotide-binding protein G(t) subunit alpha-3; Gustducin alpha-3 chain
基因编号(人):
346562;
基因编号(小鼠):
242851;
基因编号(大鼠):
286924;
蛋白编号(人):
A8MTJ3;
蛋白编号(小鼠):
Q3V3I2;
蛋白编号(大鼠):
P29348;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of GNAT3 expression in A549 (A) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 37 kD) -
Immunofluorescent analysis of GNAT3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).


说明书
MSDS