产品描述：Rabbit polyclonal antibody to GFAT2免疫原：Recombinant fusion protein of human GFAT2. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：GFPT2相关名称：Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2; D-fructose-6-phosphate amidotransferase 2; Glutamine:fructose-6-phosphate amidotransferase 2; GFAT 2; GFAT2; Hexosephosphate aminotransferase 2 基因编号(人)： 9945; 基因编号(小鼠)： 14584; 基因编号(大鼠)： 360518; 蛋白编号(人)： O94808; 蛋白编号(小鼠)： Q9Z2Z9; 蛋白编号(大鼠)： Q4KMC4; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GFAT2 expression in A375 (A), NIH3T3 (B), mouse testis (C), rat heart (D) whole cell lysates. (Predicted band size: 76 kD; Observed band size: 77 kD)
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  Immunofluorescent analysis of GFAT2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).