说明书
MSDS产品描述：Rabbit polyclonal antibody to FMO5免疫原：Recombinant fusion protein of human FMO5. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：FMO5相关名称：Dimethylaniline monooxygenase [N-oxide-forming] 5; Dimethylaniline oxidase 5; Hepatic flavin-containing monooxygenase 5; FMO 5
基因编号(人)：
2330;
基因编号(小鼠)：
14263;
基因编号(大鼠)：
246248;
蛋白编号(人)：
P49326;
蛋白编号(小鼠)：
P97872;
蛋白编号(大鼠)：
Q8K4C0;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FMO5 expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 32; 52; 60 kD; Observed band size: 60 kD) -
Immunofluorescent analysis of FMO5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.