产品描述：Rabbit polyclonal antibody to EED免疫原：KLH-conjugated synthetic peptide of human EED. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)基因名称：EED相关名称：Polycomb protein EED; hEED; WD protein associating with integrin cytoplasmic tails 1; WAIT-1 基因编号(人)： 8726; 基因编号(小鼠)： 13626; 蛋白编号(人)： O75530; 蛋白编号(小鼠)： Q921E6; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of EED expression in MCF7 (A), A549 (B), mouse lung (C) whole cell lysates. (Predicted band size: 45; 50; 53 kD; Observed band size: 50-75 kD)
• 
  Immunofluorescent analysis of EED staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.