产品描述：Rabbit polyclonal antibody to CYR61免疫原：Recombinant fusion protein of human CYR61. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：CYR61相关名称：CCN1; GIG1; IGFBP10; Protein CYR61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1 基因编号(人)： 3491; 基因编号(小鼠)： 16007; 基因编号(大鼠)： 83476; 蛋白编号(人)： O00622; 蛋白编号(小鼠)： P18406; 蛋白编号(大鼠)： Q9ES72; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CYR61 expression in A375 (A), mouse kidney (B) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 41 kD)
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  Immunofluorescent analysis of CYR61 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).