说明书
MSDS产品描述：Rabbit polyclonal antibody to NOXA免疫原：KLH-conjugated synthetic peptide of human NOXA纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：PMAIP1相关名称：NOXA; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-induced protein 1; Immediate-early-response protein APR; Protein Noxa
基因编号(人)：
5366;
基因编号(小鼠)：
58801;
基因编号(大鼠)：
492821;
蛋白编号(人)：
Q13794;
蛋白编号(小鼠)：
Q9JM54;
蛋白编号(大鼠)：
Q5U777;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NOXA expression in Jurkat (A), Hela (B), SKOV3 (C) whole cell lysates. (Predicted band size: 6; 14 kD; Observed band size: 14 kD) -
Immunofluorescent analysis of NOXA staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).