产品描述：Rabbit polyclonal antibody to CARD12免疫原：Recombinant full length protein of human CARD12纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：NLRC4相关名称：CARD12; CLAN; CLAN1; IPAF; NLR family CARD domain-containing protein 4; CARD, LRR, and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf 基因编号(人)： 58484; 基因编号(小鼠)： 268973; 基因编号(大鼠)： 298784; 蛋白编号(人)： Q9NPP4; 蛋白编号(小鼠)： Q3UP24; 蛋白编号(大鼠)： F1M649; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CARD12 expression in MCF7 (A), mouse heart (B), rat kidney (C) whole cell lysates. (Predicted band size: 116 kD; Observed band size: 110 kD)
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  Immunofluorescent analysis of CARD12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.