产品描述：Rabbit polyclonal antibody to eIF2C2免疫原：Recombinant full length protein of human eIF2C2纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：AGO2相关名称：EIF2C2; Protein argonaute-2; Argonaute2; hAgo2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; PAZ Piwi domain protein; PPD; Protein slicer 基因编号(人)： 27161; 基因编号(小鼠)： 239528; 基因编号(大鼠)： 59117; 蛋白编号(人)： Q9UKV8; 蛋白编号(小鼠)： Q8CJG0; 蛋白编号(大鼠)： Q9QZ81; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of eIF2C2 expression in Jurkat (A) whole cell lysates. (Predicted band size: 93; 97 kD; Observed band size: 110 kD)
• 
  Immunofluorescent analysis of eIF2C2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).