产品描述：Rabbit polyclonal antibody to PRPF8免疫原：KLH-conjugated synthetic peptide of human PRPF8纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)基因名称：PRPF8相关名称：PRPC8; Pre-mRNA-processing-splicing factor 8; 220 kDa U5 snRNP-specific protein; PRP8 homolog; Splicing factor Prp8; p220 基因编号(人)： 10594; 基因编号(小鼠)： 192159; 蛋白编号(人)： Q6P2Q9; 蛋白编号(小鼠)： Q99PV0; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PRPF8 expression in HepG2 (A), A431 (B) whole cell lysates. (Predicted band size: 273 kD; Observed band size: 274 kD)
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  Immunohistochemical analysis of PRPF8 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of PRPF8 staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).