说明书
MSDS产品描述：Rabbit polyclonal antibody to Granulin免疫原：Recombinant full length protein of human Granulin纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：GRN相关名称：Granulins; Proepithelin; PEPI
基因编号(人)：
2896;
基因编号(大鼠)：
29143;
蛋白编号(人)：
P28799;
蛋白编号(小鼠)：
P28798;
蛋白编号(大鼠)：
P23785;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Granulin expression in HepG2 (A), MCF7 (B), mouse liver (C), mouse kidney (D) whole cell lysates. (Predicted band size: 44; 46; 63 kD; Observed band size: 88 kD) -
Immunofluorescent analysis of Granulin staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).