产品描述：Rabbit polyclonal antibody to DNMT1免疫原：Recombinant full length protein of human DNMT1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/50 - 1/100)基因名称：DNMT1相关名称：AIM; CXXC9; DNMT; DNA (cytosine-5)-methyltransferase 1; Dnmt1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT 基因编号(人)： 1786; 基因编号(小鼠)： 13433; 蛋白编号(人)： P26358; 蛋白编号(小鼠)： P13864; 蛋白编号(大鼠)： Q9Z330; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DNMT1 expression in SW480 (A) whole cell lysates. (Predicted band size: 144; 183; 184 kD; Observed band size: 200 kD)
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  Immunofluorescent analysis of DNMT1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.