说明书
MSDS产品描述：Rabbit polyclonal antibody to E2 EPF免疫原：Recombinant full length protein of human E2 EPF纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：UBE2S相关名称：E2EPF; Ubiquitin-conjugating enzyme E2 S; E2-EPF; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S
基因编号(人)：
27338;
基因编号(小鼠)：
77891;
基因编号(大鼠)：
292588;
蛋白编号(人)：
Q16763;
蛋白编号(小鼠)：
Q921J4;
蛋白编号(大鼠)：
B5DFI8;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of E2 EPF expression in Hela (A), Jurkat (B), mouse thymus (C), rat testis (D) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 24 kD) -
Immunofluorescent analysis of E2 EPF staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).