产品描述：Rabbit polyclonal antibody to EIF4E2免疫原：Recombinant full length protein of human EIF4E2纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：EIF4E2相关名称：EIF4EL3; Eukaryotic translation initiation factor 4E type 2; eIF-4E type 2; eIF4E type 2; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E-like 3; eIF4E-like protein 4E-LP; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 3 基因编号(人)： 9470; 基因编号(小鼠)： 26987; 蛋白编号(人)： O60573; 蛋白编号(小鼠)： Q8BMB3; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EIF4E2 expression in HL60 (A), mouse kidney (B) whole cell lysates. (Predicted band size: 27; 28 kD; Observed band size: 28 kD)
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  Immunofluorescent analysis of EIF4E2 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).