说明书
MSDS产品描述：Rabbit polyclonal antibody to EIF3G免疫原：Recombinant full length protein of human EIF3G纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：EIF3G相关名称：EIF3S4; Eukaryotic translation initiation factor 3 subunit G; eIF3g; Eukaryotic translation initiation factor 3 RNA-binding subunit; eIF-3 RNA-binding subunit; Eukaryotic translation initiation factor 3 subunit 4; eIF-3-delta; eIF3 p42; eIF3 p44
基因编号(人)：
8666;
基因编号(小鼠)：
53356;
基因编号(大鼠)：
298700;
蛋白编号(人)：
O75821;
蛋白编号(小鼠)：
Q9Z1D1;
蛋白编号(大鼠)：
Q5RK09;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EIF3G expression in Jurkat (A), Hela (B), NIH3T3 (C), mouse heart (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 42 kD) -
Immunofluorescent analysis of EIF3G staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).