产品描述：Rabbit polyclonal antibody to MAD1免疫原：Recombinant full length protein of human MAD1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：MAD1L1相关名称：MAD1; TXBP181; Mitotic spindle assembly checkpoint protein MAD1; Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181 基因编号(人)： 8379; 基因编号(小鼠)： 17120; 蛋白编号(人)： Q9Y6D9; 蛋白编号(小鼠)： Q9WTX8; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MAD1 expression in Hela (A), HepG2 (B), MCF7 (C) whole cell lysates. (Predicted band size: 72; 83 kD; Observed band size: 95 kD)
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  Immunofluorescent analysis of MAD1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.