产品描述：Rabbit polyclonal antibody to P4HA1免疫原：Recombinant full length protein of human P4HA1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：P4HA1相关名称：P4HA; Prolyl 4-hydroxylase subunit alpha-1; 4-PH alpha-1; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1 基因编号(人)： 5033; 基因编号(小鼠)： 18451; 蛋白编号(人)： P13674; 蛋白编号(小鼠)： Q60715; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of P4HA1 expression in A431 (A), HEK293T (B) whole cell lysates. (Predicted band size: 58; 60; 61 kD; Observed band size: 61 kD)
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  Immunofluorescent analysis of P4HA1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).