说明书
MSDS产品描述：Rabbit polyclonal antibody to TRPV5免疫原：Recombinant full length protein of human TRPV5纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)基因名称：TRPV5相关名称：ECAC1; Transient receptor potential cation channel subfamily V member 5; TrpV5; Calcium transport protein 2; CaT2; Epithelial calcium channel 1; ECaC; ECaC1; Osm-9-like TRP channel 3; OTRPC3
基因编号(人)：
56302;
基因编号(小鼠)：
194352;
基因编号(大鼠)：
116469;
蛋白编号(人)：
Q9NQA5;
蛋白编号(小鼠)：
P69744;
蛋白编号(大鼠)：
Q9JIP0;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of TRPV5 expression in HepG2 (A), HT29 (B), mouse kidney (C) whole cell lysates. (Predicted band size: 43; 82 kD; Observed band size: 83 kD) -
Immunofluorescent analysis of TRPV5 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.