产品描述：Rabbit polyclonal antibody to NDR1免疫原：Recombinant full length protein of human NDR1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：STK38相关名称：NDR1; Serine/threonine-protein kinase 38; NDR1 protein kinase; Nuclear Dbf2-related kinase 1 基因编号(人)： 11329; 基因编号(小鼠)： 106504; 蛋白编号(人)： Q15208; 蛋白编号(小鼠)： Q91VJ4; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NDR1 expression in A549 (A), SW480 (B), 293T (C), MCF7 (D), mouse liver (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 62 kD)
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  Immunofluorescent analysis of NDR1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).