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CQA2241
  • Western blot analysis of SMARCAD1 expression in A549 (A), HepG2 (B), mouse kidney (C), mouse lung (D) whole cell lysates. (Predicted band size: 68; 117 kD; Observed band size: 120 kD)
  • Immunofluorescent analysis of SMARCAD1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
产品名称:Anti-SMARCAD1 Antibody
货号:CQA2241
来源:Rabbit
反应物种:H, M, R
实验应用:WB, IF/IC
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
3500
100 μl
2200
50 μl
1500
30 μl
1100
Ship in 3 days
产品描述:Rabbit polyclonal antibody to SMARCAD1
免疫原:Recombinant full length protein of human SMARCAD1
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
基因名称:SMARCAD1
相关名称:KIAA1122; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1; ATP-dependent helicase 1; hHEL1
基因编号(人): 56916;
基因编号(小鼠): 13990;
基因编号(大鼠): 312398;
蛋白编号(人): Q9H4L7;
蛋白编号(小鼠): Q04692;
蛋白编号(大鼠): D3Z9Z9;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of SMARCAD1 expression in A549 (A), HepG2 (B), mouse kidney (C), mouse lung (D) whole cell lysates. (Predicted band size: 68; 117 kD; Observed band size: 120 kD)
  • Immunofluorescent analysis of SMARCAD1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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