产品描述：Rabbit polyclonal antibody to SH3GL2免疫原：Recombinant full length protein of human SH3GL2纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：SH3GL2相关名称：CNSA2; SH3D2A; Endophilin-A1; EEN-B1; Endophilin-1; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2 基因编号(人)： 6456; 基因编号(小鼠)： 20404; 蛋白编号(人)： Q99962; 蛋白编号(小鼠)： Q62420; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SH3GL2 expression in Hela (A), HepG2 (B), mouse brain (C), mouse testis (D) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 39 kD)
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  Immunofluorescent analysis of SH3GL2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).