产品描述：Rabbit polyclonal antibody to HSP47免疫原：Recombinant full length protein of human HSP47纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：SERPINH1相关名称：CBP1; CBP2; HSP47; SERPINH2; Serpin H1; 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47 基因编号(人)： 871; 基因编号(小鼠)： 12406; 基因编号(大鼠)： 29345; 蛋白编号(人)： P50454; 蛋白编号(小鼠)： P19324; 蛋白编号(大鼠)： P29457; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSP47 expression in Hela (A), U251 (B), THP1 (C) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)
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  Immunofluorescent analysis of HSP47 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.