产品描述：Rabbit polyclonal antibody to P2X4免疫原：Recombinant full length protein of human P2X4纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)基因名称：P2RX4相关名称：P2X purinoceptor 4; P2X4; ATP receptor; Purinergic receptor 基因编号(人)： 5025; 基因编号(大鼠)： 29659; 蛋白编号(人)： Q99571; 蛋白编号(小鼠)： Q9JJX6; 蛋白编号(大鼠)： P51577; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of P2X4 expression in SW480 (A), THP1 (B) whole cell lysates. (Predicted band size: 40; 43; 45 kD; Observed band size: 62 kD)
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  Immunofluorescent analysis of P2X4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.