说明书
MSDS产品描述：Rabbit polyclonal antibody to DAX1免疫原：Recombinant full length protein of human DAX1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)基因名称：NR0B1相关名称：AHC; DAX1; Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1
基因编号(人)：
190;
基因编号(小鼠)：
11614;
基因编号(大鼠)：
58850;
蛋白编号(人)：
P51843;
蛋白编号(小鼠)：
Q61066;
蛋白编号(大鼠)：
P70503;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DAX1 expression in Jurkat (A), MCF7 (B), HepG2 (C), mouse spleen (D) whole cell lysates. (Predicted band size: 43; 51 kD; Observed band size: 50 kD) -
Immunofluorescent analysis of DAX1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.