说明书
MSDS产品描述：Rabbit polyclonal antibody to IL-1RA免疫原：Recombinant full length protein of human IL-1RA纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：IL1RN相关名称：IL1F3; IL1RA; Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 inhibitor; Anakinra
基因编号(人)：
3557;
基因编号(小鼠)：
16181;
基因编号(大鼠)：
60582;
蛋白编号(人)：
P18510;
蛋白编号(小鼠)：
P25085;
蛋白编号(大鼠)：
P25086;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IL-1RA expression in Hela (A), NIH3T3 (B), HL60 (C) whole cell lysates. (Predicted band size: 16; 17; 19; 20 kD; Observed band size: 18 kD) -
Immunofluorescent analysis of IL-1RA staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.