产品描述：Rabbit polyclonal antibody to GRASP65免疫原：Recombinant full length protein of human GRASP65纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：GORASP1相关名称：GOLPH5; GRASP65; Golgi reassembly-stacking protein 1; Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; GOLPH5; Golgi reassembly-stacking protein of 65 kDa; GRASP65 基因编号(人)： 64689; 蛋白编号(人)： Q9BQQ3; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GRASP65 expression in A549 (A), U937 (B), mouse kidney (C), mouse heart (D), rat liver (E) whole cell lysates. (Predicted band size: 23; 26; 46 kD; Observed band size: 65 kD)
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  Immunofluorescent analysis of GRASP65 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.