说明书
MSDS产品描述：Rabbit polyclonal antibody to EIF6免疫原：Recombinant full length protein of human EIF6纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：EIF6相关名称：EIF3A; ITGB4BP; Eukaryotic translation initiation factor 6; eIF-6; B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP)
基因编号(人)：
3692;
基因编号(小鼠)：
16418;
基因编号(大鼠)：
305506;
蛋白编号(人)：
P56537;
蛋白编号(小鼠)：
O55135;
蛋白编号(大鼠)：
Q3KRD8;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EIF6 expression in Hela (A), HepG2 (B), K562 (C) whole cell lysates. (Predicted band size: 23; 26 kD; Observed band size: 27 kD) -
Immunofluorescent analysis of EIF6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.