产品描述：Rabbit polyclonal antibody to EIF3A免疫原：Recombinant full length protein of human EIF3A纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：EIF3A相关名称：EIF3S10; KIAA0139; Eukaryotic translation initiation factor 3 subunit A; eIF3a; Eukaryotic translation initiation factor 3 subunit 10; eIF-3-theta; eIF3 p167; eIF3 p180; eIF3 p185 基因编号(人)： 8661; 基因编号(小鼠)： 13669; 基因编号(大鼠)： 292148; 蛋白编号(人)： Q14152; 蛋白编号(小鼠)： P23116; 蛋白编号(大鼠)： Q1JU68; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EIF3A expression in HepG2 (A), Jurkat (B), mouse heart (C), mouse ovary (D) whole cell lysates. (Predicted band size: 162; 166 kD; Observed band size: 180 kD)
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  Immunofluorescent analysis of EIF3A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).