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CQA1879
  • Western blot analysis of CD36 expression in mouse heart (A), mouse muscle (B), rat heart (C), rat muscle (D) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 100; 53 kD)
  • Immunofluorescent analysis of CD36 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
产品名称:Anti-CD36 Antibody
货号:CQA1879
来源:Rabbit
反应物种:H, M, R
实验应用:WB, IF/IC
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
3500
100 μl
2200
50 μl
1500
30 μl
1100
Ship in 3 days
产品描述:Rabbit polyclonal antibody to CD36
免疫原:Recombinant full length protein of human CD36
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
基因名称:CD36
相关名称:GP3B; GP4; Platelet glycoprotein 4; Fatty acid translocase; FAT; Glycoprotein IIIb; GPIIIB; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; Platelet glycoprotein IV; GPIV; Thrombospondin receptor; CD36
基因编号(人): 948;
基因编号(小鼠): 12491;
蛋白编号(人): P16671;
蛋白编号(小鼠): Q08857;
蛋白编号(大鼠): Q07969;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CD36 expression in mouse heart (A), mouse muscle (B), rat heart (C), rat muscle (D) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 100; 53 kD)
  • Immunofluorescent analysis of CD36 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
RFX1 regulates foam cell formation and atherosclerosis by mediating CD36 expression
Journal Int Immunopharmacol
IF 5.6
Application WB
Reactivity Mouse
PMID 38402833
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