产品描述：Rabbit polyclonal antibody to CCAR1免疫原：Recombinant full length protein of human CCAR1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)基因名称：CCAR1相关名称：CARP1; DIS; Cell division cycle and apoptosis regulator protein 1; Cell cycle and apoptosis regulatory protein 1; CARP-1; Death inducer with SAP domain 基因编号(人)： 55749; 基因编号(小鼠)： 67500; 蛋白编号(人)： Q8IX12; 蛋白编号(小鼠)： Q8CH18; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CCAR1 expression in Jurkat (A), HepG2 (B), A549 (C), A431 (D) whole cell lysates. (Predicted band size: 132 kD; Observed band size: 155 kD)
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  Immunofluorescent analysis of CCAR1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.