说明书
MSDS产品描述：Rabbit polyclonal antibody to APTX免疫原：Recombinant full length protein of human APTX纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)基因名称：APTX相关名称：AXA1; Aprataxin; Forkhead-associated domain histidine triad-like protein; FHA-HIT
基因编号(人)：
54840;
基因编号(小鼠)：
66408;
基因编号(大鼠)：
259271;
蛋白编号(人)：
Q7Z2E3;
蛋白编号(小鼠)：
Q7TQC5;
蛋白编号(大鼠)：
Q8K4H4;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of APTX expression in A549 (A), K562 (B), Raji (C) whole cell lysates. (Predicted band size: 5; 13; 19-40 kD; Observed band size: 40 kD) -
Immunofluorescent analysis of APTX staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.