说明书
MSDS产品描述：Rabbit polyclonal antibody to Brn-5免疫原：Recombinant full length protein of human Brn-5纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)基因名称：POU6F1相关名称：BRN5; MPOU; TCFB1; POU domain, class 6, transcription factor 1; Brain-specific homeobox/POU domain protein 5; Brain-5; Brn-5; mPOU homeobox protein
基因编号(人)：
5463;
基因编号(小鼠)：
19009;
基因编号(大鼠)：
116545;
蛋白编号(人)：
Q14863;
蛋白编号(小鼠)：
Q07916;
蛋白编号(大鼠)：
P56223;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Brn-5 expression in HepG2 (A), Raji (B), mouse liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 45 kD) -
Immunofluorescent analysis of Brn-5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.