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CQA1550
  • Western blot analysis of HEMK2 expression in SKOV3 (A), mouse heart (B), mouse brain (C) whole cell lysates. (Predicted band size: 19; 22 kD; Observed band size: 23 kD)
  • Immunofluorescent analysis of HEMK2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
产品名称:Anti-HEMK2 Antibody
货号:CQA1550
来源:Rabbit
反应物种:H, M, R
实验应用:WB, IF/IC
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
3500
100 μl
2200
50 μl
1500
30 μl
1100
Ship in 3 days
产品描述:Rabbit polyclonal antibody to HEMK2
免疫原:Recombinant full length protein of human HEMK2
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
基因名称:N6AMT1
相关名称:C21orf127; HEMK2; HemK methyltransferase family member 2; M.HsaHemK2P; N(6)-adenine-specific DNA methyltransferase 1
基因编号(人): 29104;
蛋白编号(人): Q9Y5N5;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of HEMK2 expression in SKOV3 (A), mouse heart (B), mouse brain (C) whole cell lysates. (Predicted band size: 19; 22 kD; Observed band size: 23 kD)
  • Immunofluorescent analysis of HEMK2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
The methyltransferase N6AMT1 participates in the cell cycle by regulating cyclin E levels
Journal PLoS One
IF 3.7
Application WB, KO
Reactivity Human
PMID 38394175
Cross-Reactivity of N6AMT1 Antibodies with Aurora Kinase A: An Example of Antibody-Specific Non-Specificity
Journal Antibodies (Basel)
IF 4.7
Application WB, IF, IP
Reactivity Human
PMID 38804301
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