产品描述:Rabbit polyclonal antibody to GTL3免疫原:Recombinant full length protein of human GTL3纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)基因名称:C16orf80相关名称:UPF0468 protein C16orf80; Transcription factor IIB
基因编号(人):
29105;
基因编号(小鼠):
14894;
基因编号(大鼠):
307642;
蛋白编号(人):
Q9Y6A4;
蛋白编号(小鼠):
Q8BTU1;
蛋白编号(大鼠):
Q499T7;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of GTL3 expression in SKOV3 (A), HepG2 (B), mouse liver (C), rat lung (D) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 23 kD)
-
Immunofluorescent analysis of GTL3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.