产品描述：Rabbit polyclonal antibody to PC4免疫原：Recombinant full length protein of human PC4纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)基因名称：SUB1相关名称：PC4; RPO2TC1; Activated RNA polymerase II transcriptional coactivator p15; Positive cofactor 4; PC4; SUB1 homolog; p14 基因编号(人)： 10923; 基因编号(小鼠)： 20024; 基因编号(大鼠)： 192269; 蛋白编号(人)： P53999; 蛋白编号(小鼠)： P11031; 蛋白编号(大鼠)： Q63396; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PC4 expression in HL60 (A), Jurkat (B), mouse brain (C) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 17 kD)
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  Immunofluorescent analysis of PC4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.