产品描述：Rabbit polyclonal antibody to PCMT1免疫原：Recombinant full length protein of human PCMT1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：PCMT1相关名称：Protein-L-isoaspartate(D-aspartate) O-methyltransferase; PIMT; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase 基因编号(人)： 5110; 蛋白编号(人)： P22061; 蛋白编号(小鼠)： P23506; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PCMT1 expression in HepG2 (A), A431 (B), mouse liver (C), rat brain (D), rat heart (E) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
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  Immunofluorescent analysis of PCMT1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.