说明书
MSDS产品描述：Rabbit polyclonal antibody to p47免疫原：Recombinant full length protein of human p47纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：NSFL1C相关名称：UBXN2C; NSFL1 cofactor p47; UBX domain-containing protein 2C; p97 cofactor p47
基因编号(人)：
55968;
基因编号(小鼠)：
386649;
基因编号(大鼠)：
83809;
蛋白编号(人)：
Q9UNZ2;
蛋白编号(小鼠)：
Q9CZ44;
蛋白编号(大鼠)：
O35987;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p47 expression in mouse brain (A), rat kidney (B) whole cell lysates. (Predicted band size: 28; 37; 40 kD; Observed band size: 41 kD) -
Immunofluorescent analysis of p47 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.