说明书
MSDS产品描述：Rabbit polyclonal antibody to Int11免疫原：Recombinant full length protein of human Int11纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)基因名称：CPSF3L相关名称：INTS11; RC68; Integrator complex subunit 11; Int11; Cleavage and polyadenylation-specific factor 3-like protein; CPSF3-like protein; Protein related to CPSF subunits of 68 kDa; RC-68
基因编号(人)：
54973;
基因编号(小鼠)：
71957;
基因编号(大鼠)：
298688;
蛋白编号(人)：
Q5TA45;
蛋白编号(小鼠)：
Q9CWS4;
蛋白编号(大鼠)：
Q3MHC2;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Int11 expression in SKOV3 (A), MCF7 (B), mouse spleen (C), rat liver (D) whole cell lysates. (Predicted band size: 56; 64; 65; 67; 68 kD; Observed band size: 68 kD) -
Immunofluorescent analysis of Int11 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.